False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients
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چکیده
منابع مشابه
Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.
We report the prevalence in Bordetella bronchiseptica of IS481, a frequent target for diagnosis of Bordetella pertussis, as approximately 5%. However, PCR amplicons of the predicted size were detectable in 78% of IS481-negative strains. Our results suggest that PCR targeting IS481 may not be sufficiently specific for reliable identification of B. pertussis.
متن کاملRecovery of Bordetella pertussis from PCR-positive nasopharyngeal samples is dependent on bacterial load.
Viable Bordetella pertussis isolates are essential for surveillance purposes. We performed culture of 223 PCR-positive nasopharyngeal samples. B. pertussis was recovered from 45 (20.2%) of the samples. Growth was associated with a high bacterial load, as determined by PCR. Culture from PCR-positive samples is a feasible approach to recover B. pertussis isolates, and culture can be limited to sa...
متن کاملHarmonization of Bordetella pertussis real-time PCR diagnostics in the United States in 2012.
Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC developed a multitarget PCR assay to differentiate B. pertussis, B. holmesii, and B...
متن کاملUse of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.
This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.
متن کاملDifferential Expression of Type III Effector BteA Protein Due to IS481 Insertion in Bordetella pertussis
BACKGROUND Bordetella pertussis is the primary etiologic agent of the disease pertussis. Universal immunization programs have contributed to a significant reduction in morbidity and mortality of pertussis; however, incidence of the disease, especially in adolescents and adults, has increased in several countries despite high vaccination coverage. During the last three decades, strains of Bordet...
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ژورنال
عنوان ژورنال: The Open Microbiology Journal
سال: 2019
ISSN: 1874-2858
DOI: 10.2174/1874285801913010051